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In 3c-1 the cells are sedimented as a pellet and then resuspended in the solution of the required density, while in 3c-2 this pelleting step is omitted and the suspension mixed with a high-density solution

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One characteristic of renal cell death and a potential contributor to gentamicin-induced ARF is caspase-3, an essential component of programmed cell death (apoptosis) 88