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Primary antibodies were diluted in TBST skimmed milk 5% buffer and the incubation was performed overnight at 4°C using an orbital shaker followed by 2 h at room temperature with the secondary horseradish peroxidase (HRP)-linked antibody (Goat Anti-Mouse or Anti-Rabbit IgG Peroxidase Conjugated by Thermo Scientific)

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Inclusions:Trial Set

People worry that taking 20 grams of protein, 10 grams of collagen, and 5 grams of creatine will "overload" the system