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The statistical analysis of the lipophilic fraction (Fig 2B and B′) generated a weak OPLS-DA model with only limited class separation (with parameters R 2 = 0.56 and Q 2 = 0.28, CV-ANOVA test, P = 0.6), with a single metabolite (glycerophospholipids–sphingomyelin) responsible for the class distinction (Figs 2B, B′, and C and S3)