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Slides were then rinsed in tap water and the nuclei were counter stained with bluing reagent (ThermoFisher Scientific), rinsed in 70% ethanol, stained with eosin Y, alcoholic (ThermoFisher Scientific), then dehydrated and cleared through graded alcohols and xylene and mounted with Micromount (Leica 3801731) using a Leica CV5030 automatic coverslipper
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It follows from the process of MGO synthesis that in anaerobic glycolysis, it represents a diversion from ATP synthesis and, thus, energy generation does not occur, while preserving the possibility of a hexose monophosphate shunt for NADPH formation (Figure 2)
