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6.2.3 Gene therapy In aged mouse tibialis anterior muscle, overexpression of PGC-1α via in vivo DNA transfection markedly attenuated the age-related surge in mitophagy and fission markers, thus reducing PINK1 and Parkin levels by over 50%, lowering mitochondrial ubiquitination, and blunting increases in LC3, p62, Beclin-1 (Becn1, Atg6), Rheb, Mfn2, Fis1, and Drp1
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This pattern of accelerating improvement is consistent with the hair growth cycle — new follicles need multiple growth cycles to produce visible improvement
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