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The reaction catalyzed by γ-ECS/GSH1 is considered the rate-controlling step of GSH synthesis, and the activity of this enzyme is regulated by cellular levels of cysteine and glutamic acid, and feedback inhibition by γ-EC and GSH ( Figure 1 GSH1 (EC 6.3.2.2) is exclusively present in plastids, while GSH2 (EC 6.3.2.2) has dual localization in plastids and the cytosol, both encoded by a single gene (Wachter et al., 2005)
