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In the second PCR, 3 µL of the first PCR reaction product was used as the template and was combined with 2U of Taq, 1 × buffer, 1.5 mM MgCl 2 , 0.4 mg/mL BSA, 0.2 mM dNTPs, and 5pmols each of Illumina adapted primers 341F (CCTACGGGAGGCAGCAG) and 518R (ATTACCGCGGCTGCTGG) (primers + Illumina adapters/barcode/priming region as described in supplemental materials of Bartram et al., 2011: ~ 80 bp) 44,45,46
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The role of diet in gout prevention Dietary control may be sufficient in a patient with mildly elevated uric acid, for example, 7.0 mg/dL (noting that any uric acid level above 6.0 is considered elevated for a patient with gout, even if within what the lab calls the “normal range.”) For those with a higher level, for example, 10.0 mg/dL, diet alone will not usually prevent gout
