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For detecting the HA-DELE1 expression, the HEK293T-HA-DELE1 cells were lysed in lysis buffer (150 mM NaCl, 50 mM HEPES pH 7.5 and 1% NP-40 substitute) supplemented with Roche complete protease inhibitor cocktail, PhosSTOP phosphatase inhibitor cocktail, and benzonase (EMD Millipore, 70746-4) on ice
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